Tag Archives: Uptake

Detection and Characterization of a Mycobacterial L-Arabinofuranose ABC Transporter Identified with a Rapid Lipoproteomics Protocol

Miaomiao Li, Christoph Müller, Klemens Fröhlich, Oliver Gorka, Lin Zhang, Olaf Groß, Oliver Schilling, Oliver Einsle, Claudia Jessen-Trefzer

Nutrient uptake is essential for survival of organisms, and carbohydrates serve as a crucial carbon and energy source for most microorganisms. Given the importance of mycobacteria as human pathogens a detailed knowledge of carbohydrate uptake transporters is highly desirable, but currently available information is severely limited and mainly based on in silico analyses. Read more

Construction of Bacterial Cells with an Active Transport System for Unnatural Amino Acids

Wooseok Ko, Rahul Kumar, Sanggil Kim, and Hyun Soo Lee

Engineered organisms with an expanded genetic code have attracted much attention in chemical and synthetic biology research. In this work, engineered bacterial organisms with enhanced unnatural amino acid (UAA) uptake abilities were developed by screening periplasmic binding protein (PBP) mutants for recognition of UAAs. Read more