Daniela Heeg – CHAIN Biotech
Technical Product Manager
What is your background and current job role?
I obtained a PhD in Molecular Medical Microbiology from the University of Nottingham, where I undertook a project concerned with the spore formation and spore germination of the important human pathogen Clostridium difficile. Following this, I worked at the University of Nottingham as Postdoctoral researcher and in clinical diagnostics at a private company before joining CHAIN Biotechnology Ltd as Technical Product Manager. Here, I am responsible for the development and dissemination of our product range, including commercial tools such as the modular pMTL80000 vector series and the first therapeutic products in our pipeline.
What Industrial Biotechnology and Bioenergy (IBBE) related project is currently being undertaken by your organisation?
Currently, we are using Clostridium spp. as chassis to secrete therapeutic substances for the treatment of inflammatory and infectious bowel diseases. We have produced our first genetically modified strain secreting therapeutic, CHN-1, in volumes to support early in vitro pre-clinical work. We are now investigating in scale-up of this and other strains to improve growth. We are also researching an inducible version of spore production.
What do you think the challenges related to this project are in the next 1-5 years?
As CHAIN identify novel therapeutic targets, methods of secretion for novel peptides in Clostridium will need to be developed. We currently have a collaboration with the University of Nottingham in this area. In addition, because we are using the spores of our strain in formulation, we cannot induce spore formation with any substance that would prevent us from using the resulting spores in human clinical trials and subsequently in medicine. Thus, we cannot induce using common systems such as antibiotic inducible system. We also have the need for a truly tight system, so any system that can be triggered by external natural substances is not ideal for our purpose.
How can other CBMNet members help you and your organisation with your research?
Other CBMNet members could help us with our research by suggesting and maybe testing systems in the scope of an interaction voucher or more substantial funding. Such projects could focus on identification or secretion of peptides from bacteria or induction of sporulation that would be acceptable for deliberate release of an organism.