Papers published from CBMNet projects

Below you can view papers that have been generated through CBMNet funded projects…

General calibration of microbial growth in microplate readers – Keiran Stevenson, Alexander F. McVey, Ivan B. N. Clark, Peter S. Swain & Teuta Pilizota

Including reference to CBMNet funded Vacation Scholarship and Proof-of-Concept projects

Optical density (OD) measurements of microbial growth are one of the most common techniques used in microbiology, with applications ranging from studies of antibiotic efficacy to investigations of growth under different nutritional or stress environments, to characterization of different mutant strains, including those harbouring synthetic circuits. OD measurements are performed under the assumption that the OD value obtained is proportional to the cell number, i.e. the concentration of the sample. However, the assumption holds true in a limited range of conditions, and calibration techniques that determine that range are currently missing. Here we present a set of calibration procedures and considerations that are necessary to successfully estimate the cell concentration from OD measurements.

Dynamics of Escherichia coli’s passive response to a sudden decrease in external osmolarity – Renata Budaa, Yunxiao Liub, Jin Yangb, Smitha Hegdea, Keiran Stevensona, Fan Baib and Teuta Pilizota

Including reference to CBMNet funded Vacation Scholarship and Proof-of-Concept projects

Mechanosensation is central to life. Bacteria, like the majority of walled cells, live and grow under significant osmotic pressure. By relying on mechanosensitive regulation, bacteria can adapt to dramatic changes in osmotic pressure. Studying such mechanical sensing and control is critical for understanding bacterial survival in a complex host and natural environment. Here, we investigate the fundamental design principles of Escherichia coli’s passive mechanosensitive response to osmotic downshocks by implementing single-cell high-resolution imaging. We explain the observed cell volume changes by modeling flux of water and solutes across the cell membrane. A better characterization of bacterial mechanosensitive response can help us map their reaction to environmental threats.

Biosurfactants – E-book (Frontiers in Microbiology) – Pattanathu K.S.M. Rahman

Including reference to CBMNet funded Proof-of-Concept project ‘Plants as Nanoparticle Producers’

Covers a compilation of original research articles, reviews and research commentary submitted by researchers enthusiastically working in the field of biosurfactants and highlights recent advances in our knowledge of the biosurfactants and understanding of the biochemical and molecular mechanisms involved in their production, scale-up and industrial applications. There are 11 manuscripts accepted for publication in this research topic contributed by 55 authors from UK, Denmark, Greece, Germany, South Africa, India, Brazil, Bahrain, Portugal, and China.