Wooseok Ko, Rahul Kumar, Sanggil Kim, and Hyun Soo Lee
Engineered organisms with an expanded genetic code have attracted much attention in chemical and synthetic biology research. In this work, engineered bacterial organisms with enhanced unnatural amino acid (UAA) uptake abilities were developed by screening periplasmic binding protein (PBP) mutants for recognition of UAAs. (read more…)
Kenta Tsutsumi, Ryo Yonehara, Etsuko Ishizaka-Ikeda, Naoyuki Miyazaki, Shintaro Maeda, Kenji Iwasaki, Atsushi Nakagawa & Eiki Yamashita
In Pseudomonas aeruginosa, MexAB–OprM plays a central role in multidrug resistance by ejecting various drug compounds, which is one of the causes of serious nosocomial infections. (read more…)
Nuria Gómez-Santos, Timo Glatter, Ralf Koebnik, Magdalena Anna Świątek-Połatyńska & Lotte Søgaard-Andersen
TonB-dependent transporters (TBDTs) are ubiquitous outer membrane β-barrel proteins that import nutrients and bacteriocins across the outer membrane in a proton motive force-dependent manner, by directly connecting to the ExbB/ExbD/TonB system in the inner membrane. (read more…)
Alexander Jussupow, Andrea Di Luca and Ville R. I. Kaila
Cardiolipin modulates the activity of membrane-bound respiratory enzymes that catalyze biological energy transduction. The respiratory complex I functions as the primary redox-driven proton pump in mitochondrial and bacterial respiratory chains, and its activity is strongly enhanced by cardiolipin. (read more…)
Alvin C. K. Teo, Sarah C. Lee, Naomi L. Pollock, Zoe Stroud, Stephen Hall, Alpesh Thakker, Andrew R. Pitt, Timothy R. Dafforn, Corinne M. Spickett & David I. Roper
Biological characterisation of membrane proteins lags behind that of soluble proteins. This reflects issues with the traditional use of detergents for extraction, as the surrounding lipids are generally lost, with adverse structural and functional consequences. (read more…)
Production of industrial chemicals using renewable biomass feedstock is becoming increasingly important to address limited fossil resources, climate change and other environmental problems. (read more…)
The evolution of gene fusions that result in covalently linked protein domains is widespread in bacteria, where spatially coupling domain functionalities can have functional advantages in vivo.Fusions to integral membrane proteins are less widely studied but could provide routes to enhance membrane function in synthetic biology. (read more…)
A recent publication from CBMNet member Graham Stafford at the University of Sheffield. The research in this article was supported by a Proof-of-Concept Funding and by a Business Interaction Voucher from CBMNet (follow the links to read the case studies) and was part of a long-standing collaboration with Fujifilm Diosynth Biotechnologies. (read more…)
The microbial membrane serves as a biological barrier that separates the interior of cells from the external environment, thus playing an important role in tolerance to stress conditions during industrial bioprocessing. (read more…)
Corynebacterium glutamicum has been mainly used for industrial production of amino acids, and in recent years, it has also been successfully engineered to broaden its range of substrate and product profiles. (read more…)
The only known source of vitamin B12 (adenosylcobalamin) is from bacteria and archaea. Here, using genetic and metabolic engineering, we generate an Escherichia coli strain that produces vitamin B12 via an engineered de novo aerobic biosynthetic pathway. (read more…)
Thomas Eng, Philipp Demling, Robin A. Herbert, Yan Chen, Veronica Benites, Joel Martin, Anna Lipzen, Edward E. K. Baidoo, Lars M. Blank, Christopher J. Petzold, Aindrila Mukhopadhyay
Microbial production of chemicals from renewable carbon sources enables a sustainable route to many bioproducts. Sugar streams, such as those derived from biomass pretreated with ionic liquids (IL), provide efficiently derived and cost-competitive starting materials. A limitation to this approach is that residual ILs in the pretreated sugar source can be inhibitory to microbial growth and impair expression of the desired biosynthetic pathway.
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Endosymbiotic theory suggests that mitochondria evolved from free-living prokaryotes which entered the host cell and were retained as endosymbionts. Here, we model this earliest stage of the endosymbiotic theory of mitochondrial evolution by engineering endosymbiosis between two genetically tractable model organisms, Escherichia coli and Saccharomyces cerevisiae. (read more…)
Itay Budin, Tristan de Rond, Yan Chen, Leanne Jade G. Chan, Christopher J. Petzold, Jay D. Keasling
Lipid composition determines the physical properties of biological membranes and can vary substantially between and within organisms. We describe a specific role for the viscosity of energy-transducing membranes in cellular respiration. (read more…)
Emmanuel Nji, Yurie Chatzikyriakidou, Michael Landreh & David Drew
Membrane bilayers are made up of a myriad of different lipids that regulate the functional activity, stability, and oligomerization of many membrane proteins. Despite their importance, screening the structural and functional impact of lipid–protein interactions to identify specific lipid requirements remains a major challenge. (read more…)
Royal Society Publishing has recently published a special issue of Interface Focus entitled “The artificial cell: biology-inspired compartmentalization of chemical function”, organised by Paul A Beales, Barbara Ciani and Stephen Mann.
This issue is based on a Royal Society Theo Murphy meeting held on the 26–27 February 2018. The articles reveal the rich diversity of research currently being undertaken in the field of artificial cell design and construction, and highlight the challenges that lie ahead.
The articles are FREE TO ACCESS here.
CBMNet-funded scientists from the Universities of York and Oxford, along with industrial partner Unilever, have unravelled a key part of the molecular process by which armpit bacteria produce the most pungent component of the noxious smell we recognise as BO. The findings could result in more effective deodorants with targeted active ingredients, the researchers suggest. (read more…)
Protein translocation is an essential feature of cellular organisms. Bacteria, like all single-cell organisms, interact with their environment by translocation of proteins across their cell membranes via dedicated secretion pathways. Proteins destined for secretion are directed toward the secretion pathways by the presence of specific signal peptides. This study demonstrates that under conditions of both osmotic stress and translation stress, E. coli cells undergo an excretion phenomenon whereby signal peptide-less proteins are translocated across both the inner and outer cell membranes into the extracellular environment. Confirming the presence of alternative translocation/excretion pathways and understanding their function and regulation are thus important for fundamental microbiology and biotechnology. (read more…)