Easy regulation of metabolic flux in Escherichia coli using an endogenous type I-E CRISPR-Cas system

Easy regulation of metabolic flux in Escherichia coli using an endogenous type I-E CRISPR-Cas system

Clustered regularly interspaced short palindromic repeats interference (CRISPRi) is a recently developed powerful tool for gene regulation. In Escherichia coli, the type I CRISPR system expressed endogenously shall be easy for internal regulation without causing metabolic burden in compared with the widely used type II system, which expressed dCas9 as an additional plasmid.

 

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