Easy regulation of metabolic flux in Escherichia coli using an endogenous type I-E CRISPR-Cas system

Yizhao Chang, Tianyuan Su, Qingsheng Qi and Quanfeng Liang

Clustered regularly interspaced short palindromic repeats interference (CRISPRi) is a recently developed powerful tool for gene regulation. In Escherichia coli, the type I CRISPR system expressed endogenously shall be easy for internal regulation without causing metabolic burden in compared with the widely used type II system, which expressed dCas9 as an additional plasmid.


Read the full article in Microbial Cell Factories

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